TY - BOOK ED - United Nations Educational, Scientific and Cultural Organization TI - Promtion of sustainable utilization and conservation of endangered medicinal plants in mount Elgon and Metu forest reserves through evidence-based plant-parts substitution SN - 9789970400454 U1 - 581.68096761 23 rd ed. PY - 2018/// CY - Uganda PB - Uganda National Commission for UNESCO KW - Medicinal - - plant N1 - Chapter one. 1.0 General introduction - - 1.1 Statement of the problem and justification - - 1.2 Objectives - - 1.2.1 Main objective - - 1.2.2 Specific objectives - - Methodology - - 1.3 Survey on endangered medicinal plants in Metu and Mt Elgon Biosphere Reserves - - 1.3.1 Geographical background of the study areas - - 1.3.2 Key informant interviews and plant collection - - 1.3.3. Focused group discussions - - 14 Data analysis and quantitative Ethnobotany - - 1.5 Results and Discussion - - 1.5.1 Demographic characteristics of respondents and indigenous knowledge on medicinal plants - - 1.6 Fidelity level of the commonly used plants - - 1.7 Plant parts and growth forms used for medicinal purposes - - 1.8 Herbal remedy preparation, administration and storage - - 1.9 Source of traditional knowledge skills and experience - - 1.10 Modern health practitioners' view on the use of herbal medicine - - 1.1| Availability, conservation status and threats to medicinal plant species - - 1.12 Responses on solutions to challenges faced by the traditional healers - - 1.13 Menmbership to any traditional healers' association - - 1.14 Limitations of the study - - 1.15 Conclusions and Recommendations - - 1.15.1 Conclusions - - 1.15.2 Recommendations - - CHAPTER TWO... 2.0. Extraction, identification, quantification and comparison of active components in the stem bark and leaf powders of P. africana and Olea welwitschii - - 2.1 Introduction - - 2.2 Materials and Methods - - 2.2.1 Hot maceration - - 2.2.2 Phytochemical screening - - 2.3 Isolation of crude saponins fronm aqueous extracts of Prunus Africana - - 2.4 Comparison of total saponins from P. africana leaf and stem bark using UV spectroscopy - - 2.5 Isolation of crude saponins from crude aqueous stem bark and leaf extracts of Elgon teak - - CHAPTER THREE. 37 - - 3.1 Toxicity profile of aqueous bark crude extracts of P. africana and O. wehvitschii in mice - - 6.1.1 Materials and Methods - - 3.1.2 Experimental design - - 3.1.3 Histopathological investigation of acute and sub-acute toxicity of the extracts - - 3.2 Results and discussion - - 3.2.1. Acute toxicity signs of P. africana stem bark and leaf extracts in mice - - 3.2.2 Acute toxicity signs of Olea welwitschii in mice - - 3.2.3 Histopathological picture of acute oral exposure to P. africana bark extract - - 3.2.4 Histopathological picture of acute oral exposure to O. welwitschii bark extract - - 3.2.5 Sub-acute toxic effect of oral exposure to sten bark extract of P. Africana - - 3.2.5.1 Effect of sub-acute exposure on level of testosterone - - 3.2.5.2. Effect of sub-acute oral exposure to P. africana stem bark extract onbio chemical - - parameters.. 3.2.5.3 Sub-acute toxicity histopathological lesions after oral exposure to the stem bark extract of P. africana - - 3.2.54 Histopathological changes in the testicular tissue following acute and sub-acute oral exposure to aqueous extracts of P. africana. 5 - - CHAPTER FOUR - - 4.1 Antibacterial and antifungal activity of aqueous extract of Olea welwitschi - - 4.2 Materials and Methods - - 4.2.1 Antimicrobial susceptibility test - - 4.2.2 Determination of Minimum inhibitory concentration of the extracts - - 4.2.3 Data analysis - - 4.3 Results and discussion - - 4.3.1 Antibacterial and antifungal activity of the stem bark extract of Elgon teak - - 4.3.2 Minimum inhibitory concentration (MIC) - - 4.3.3 Minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFO) of the aqueous extracts of Elgon teak tree - - ER -