Chapter one.
1.0 General introduction - -
1.1 Statement of the problem and justification - -
1.2 Objectives - -
1.2.1 Main objective - -
1.2.2 Specific objectives - -

Methodology - -
1.3 Survey on endangered medicinal plants in Metu and Mt Elgon Biosphere Reserves - -
1.3.1 Geographical background of the study areas - -
1.3.2 Key informant interviews and plant collection - -
1.3.3. Focused group discussions - -
14 Data analysis and quantitative Ethnobotany - -

1.5 Results and Discussion - -
1.5.1 Demographic characteristics of respondents and indigenous knowledge on medicinal
plants - -
1.6 Fidelity level of the commonly used plants - -
1.7 Plant parts and growth forms used for medicinal purposes - -
1.8 Herbal remedy preparation, administration and storage - -
1.9 Source of traditional knowledge skills and experience - -
1.10 Modern health practitioners' view on the use of herbal medicine - -
1.1| Availability, conservation status and threats to medicinal plant species - -
1.12 Responses on solutions to challenges faced by the traditional healers - -
1.13 Menmbership to any traditional healers' association - -
1.14 Limitations of the study - -
1.15 Conclusions and Recommendations - -
1.15.1 Conclusions - -
1.15.2 Recommendations - -
CHAPTER TWO...
2.0. Extraction, identification, quantification and comparison of active components in the stem
bark and leaf powders of P. africana and Olea welwitschii - -
2.1 Introduction - -
2.2 Materials and Methods - -
2.2.1 Hot maceration - -
2.2.2 Phytochemical screening - -
2.3 Isolation of crude saponins fronm aqueous extracts of Prunus Africana - -
2.4 Comparison of total saponins from P. africana leaf and stem bark using UV
spectroscopy - -
2.5 Isolation of crude saponins from crude aqueous stem bark and leaf extracts of Elgon teak - -
CHAPTER THREE. 37 - -
3.1 Toxicity profile of aqueous bark crude extracts of P. africana and O. wehvitschii in mice - -
6.1.1 Materials and Methods - -
3.1.2 Experimental design - -

3.1.3 Histopathological investigation of acute and sub-acute toxicity of the extracts - -
3.2 Results and discussion - -
3.2.1. Acute toxicity signs of P. africana stem bark and leaf extracts in mice - -
3.2.2 Acute toxicity signs of Olea welwitschii in mice - -
3.2.3 Histopathological picture of acute oral exposure to P. africana bark extract - -
3.2.4 Histopathological picture of acute oral exposure to O. welwitschii bark extract - -
3.2.5 Sub-acute toxic effect of oral exposure to sten bark extract of P. Africana - -
3.2.5.1 Effect of sub-acute exposure on level of testosterone - -
3.2.5.2. Effect of sub-acute oral exposure to P. africana stem bark extract onbio chemical - -
parameters..
3.2.5.3 Sub-acute toxicity histopathological lesions after oral exposure to the stem bark extract of P. africana - -

3.2.54 Histopathological changes in the testicular tissue following acute and sub-acute oral exposure to aqueous extracts of P. africana. 5 - -

CHAPTER FOUR - -
4.1 Antibacterial and antifungal activity of aqueous extract of Olea welwitschi - -
4.2 Materials and Methods - -
4.2.1 Antimicrobial susceptibility test - -
4.2.2 Determination of Minimum inhibitory concentration of the extracts - -
4.2.3 Data analysis - -
4.3 Results and discussion - -
4.3.1 Antibacterial and antifungal activity of the stem bark extract of Elgon teak - -
4.3.2 Minimum inhibitory concentration (MIC) - -
4.3.3 Minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFO) of the aqueous extracts of Elgon teak tree - -